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Issue 17, 2013
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LC–ESI–MS/MS study of carvedilol and its stress degradation products

C. Purna Chander,a  B. Raju,a  Asmath Sulthanaa  and  R. Srinivas*a 
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* Corresponding authors

a National Centre for Mass Spectrometry, Indian Institute of Chemical Technology, Hyderabad-500007, India
E-mail: srini@iict.res.in, sragampeta@yahoo.co.in
Fax: +91-40-27193156
Tel: +91-40-27193482

Abstract

An isocratic liquid chromatography–electrospray ionization-mass spectrometry (LC–ESI–MS) method has been developed for the separation and identification of stress degradation products (DPs) of carvedilol (CAR), a non-selective β-blocker with vasodilatory properties. CAR was subjected to hydrolytic (acidic, alkaline and neutral), oxidative, photolytic and thermal stress conditions as per ICH guidelines Q1A (R2). The drug degraded under acidic, basic and photolytic stress conditions while it was stable under neutral, thermal and oxidative stress conditions. A total of five DPs were formed which were all well resolved from the parent drug as well as from each other on an Agilent XDB C-18 column (4.6 × 150 mm, 5 μm) using a mobile phase comprising of 20 mM ammonium acetate (pH adjusted to 6) (solvent A) and acetonitrile (solvent B) in an isocratic elution method. The structures of the degradation products were elucidated by using LC–ESI–MS/MS combined with accurate mass measurements.

Graphical abstract: LC–ESI–MS/MS study of carvedilol and its stress degradation products

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Publication details

The article was received on 14 May 2013, accepted on 28 Jun 2013 and first published on 02 Jul 2013


Article type: Communication
DOI: 10.1039/C3AY40804C
Citation: Anal. Methods, 2013,5, 4330-4335
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    LC–ESI–MS/MS study of carvedilol and its stress degradation products

    C. P. Chander, B. Raju, A. Sulthana and R. Srinivas, Anal. Methods, 2013, 5, 4330
    DOI: 10.1039/C3AY40804C

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