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Issue 17, 2013
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Application of free radical diphenylpicrylhydrazyl (DPPH) to estimate the antioxidant capacity of food samples

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Abstract

The 2,2-diphenylpicrylhydrazyl (DPPH) assay is widely used in plant biochemistry to evaluate the properties of plant constituents for scavenging free radicals. The method is based on the spectrophotometric measurement of the DPPH concentration change resulting from the reaction with an antioxidant. Several protocols have been followed for this assay using different conditions such as different reaction times, solvents, pH and different compounds used as antioxidant standards. This review shows to what extent the mentioned parameters have the influence on the presented results.

Graphical abstract: Application of free radical diphenylpicrylhydrazyl (DPPH) to estimate the antioxidant capacity of food samples

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Publication details

The article was received on 07 Mar 2013, accepted on 11 Jun 2013 and first published on 11 Jun 2013


Article type: Minireview
DOI: 10.1039/C3AY40367J
Citation: Anal. Methods, 2013,5, 4288-4295
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    Application of free radical diphenylpicrylhydrazyl (DPPH) to estimate the antioxidant capacity of food samples

    K. Pyrzynska and A. Pękal, Anal. Methods, 2013, 5, 4288
    DOI: 10.1039/C3AY40367J

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