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Issue 13, 2013
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A portable, pressure driven, room temperature nucleic acid extraction and storage system for point of care molecular diagnostics

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Abstract

Many new and exciting portable HIV viral load testing technologies are emerging for use in global medicine. While the potential to provide fast, isothermal, and quantitative molecular diagnostic information to clinicians in the field will soon be a reality, many of these technologies lack a robust front end for sample clean up and nucleic acid preparation. Such a technology would enable many different downstream molecular assays. Here, we present a portable system for centrifuge-free room temperature nucleic acid extraction from small volumes of whole blood (70 μL), using only thermally stable reagents compatible with storage and transport in low resource settings. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis of simulated samples demonstrate a lower limit of detection of 1000 copies per mL, with the ability to detect differences in viral load across four orders of magnitude. The system can also be used to store extracted RNA on detachable cartridges for up to one week at ambient temperature, and can be operated using only hand generated air pressure.

Graphical abstract: A portable, pressure driven, room temperature nucleic acid extraction and storage system for point of care molecular diagnostics

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Publication details

The article was received on 28 Jan 2013, accepted on 11 May 2013 and first published on 13 May 2013


Article type: Paper
DOI: 10.1039/C3AY40162F
Citation: Anal. Methods, 2013,5, 3177-3184
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    A portable, pressure driven, room temperature nucleic acid extraction and storage system for point of care molecular diagnostics

    S. Byrnes, A. Fan, J. Trueb, F. Jareczek, M. Mazzochette, A. Sharon, A. F. Sauer-Budge and C. M. Klapperich, Anal. Methods, 2013, 5, 3177
    DOI: 10.1039/C3AY40162F

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