Lipid hydroxide determination on a ferrocenemethanol modified electrode

Abstract

The main products generated by lipid peroxidation are the lipid hydroperoxides, compounds that are involved in the pathology of several diseases and in the process of food rancidification. In this context, simple, fast, and reliable analytical tools for the determination of lipid hydroperoxides are highly required. The present work describes the development of a single use ferrocenemethanol modified carbon screen-printed electrode for the determination of lipid hydroperoxides. The modified electrode is equally applicable to evaluation of peroxidation inhibition degree of two well-known antioxidants. The performance characteristics of the proposed non-enzymatic sensor are: linear working range 6.0 × 10⁻⁶ to 2.7 × 10⁻⁴ mol L⁻¹, limit of detection 1.7 × 10⁻⁶ mol L⁻¹, upper control limit 3 × 10⁻⁴ mol L⁻¹ and sensitivity 826.53 nA mM⁻¹; the required time to reach the steady state was 60 s. The sensor was then applied for the determination of lipid hydroperoxide formation from four real samples of vegetal oils, walnut, sunflower, linseed and soybean oils, the results being expressed as linoleic acid equivalents. Furthermore, the efficacy of Trolox and butylated hydroxyanisole in inhibiting lipid hydroperoxide formation was tested.