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Issue 2, 2013
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TanA: a fluorogenic probe for thiaminase activity

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Abstract

A fluorogenic thiamine analogue is presented as a fluorescent probe for thiaminase activity. The emission of the fluorophore is quenched by photoinduced electron transfer (PET) to the N-substituted pyridinium portion of the probe. Action of the enzyme releases the free pyridine group causing a substantial increase in fluorescence.

Graphical abstract: TanA: a fluorogenic probe for thiaminase activity

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Publication details

The article was received on 15 Sep 2012, accepted on 27 Nov 2012 and first published on 13 Dec 2012


Article type: Communication
DOI: 10.1039/C2AY26047F
Citation: Anal. Methods, 2013,5, 446-448
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    TanA: a fluorogenic probe for thiaminase activity

    W. Zhu, J. L. Zajicek, D. E. Tillitt and T. E. Glass, Anal. Methods, 2013, 5, 446
    DOI: 10.1039/C2AY26047F

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