Issue 2, 2013
From the journal:

Analytical Methods

TanA: a fluorogenic probe for thiaminase activity†

Wanjun Zhu,a   James L. Zajicek,b   Donald E. Tillittb  and  Timothy E. Glass*a  

* Corresponding authors

a University of Missouri, 125 Chemistry, 601 S. College Ave., Columbia, MO, USA
E-mail: GlassT@missouri.edu
Fax: +1 573 882 2754
Tel: +1 573 882 3813

b U.S. Geological Survey, Columbia Environmental Research Center, 4200 New Haven Road, Columbia, Missouri, USA
E-mail: jzajicek@usgs.gov
Fax: +1 573 876 1896
Tel: +1 573 876 1885

Abstract

A fluorogenic thiamine analogue is presented as a fluorescent probe for thiaminase activity. The emission of the fluorophore is quenched by photoinduced electron transfer (PET) to the N-substituted pyridinium portion of the probe. Action of the enzyme releases the free pyridine group causing a substantial increase in fluorescence.

Graphical abstract: TanA: a fluorogenic probe for thiaminase activity

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Article information

DOI
https://doi.org/10.1039/C2AY26047F
Article type
Communication
Submitted
15 Sep 2012
Accepted
27 Nov 2012
First published
13 Dec 2012

Anal. Methods, 2013,5, 446-448

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TanA: a fluorogenic probe for thiaminase activity

W. Zhu, J. L. Zajicek, D. E. Tillitt and T. E. Glass, Anal. Methods, 2013, 5, 446 DOI: 10.1039/C2AY26047F

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