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Issue 24, 2013
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Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers

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Abstract

Single-channel electrophysiology with lipid bilayer systems requires ion channel expression, purification from cell culture, and reconstitution in proteoliposomes for delivery to a planar bilayer. Here we demonstrate that single-channel current measurements of the potassium channels KcsA and hERGS5–S6 can be obtained by direct insertion in interdroplet lipid bilayers from microliters of a cell-free expression medium.

Graphical abstract: Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers

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Publication details

The article was received on 13 Aug 2013, accepted on 18 Oct 2013 and first published on 18 Oct 2013


Article type: Communication
DOI: 10.1039/C3AN01540H
Citation: Analyst, 2013,138, 7294-7298
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    Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers

    M. S. Friddin, N. P. Smithers, M. Beaugrand, I. Marcotte, P. T. F. Williamson, H. Morgan and M. R. R. de Planque, Analyst, 2013, 138, 7294
    DOI: 10.1039/C3AN01540H

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