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Issue 19, 2013
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Combined shifted-excitation Raman difference spectroscopy and support vector regression for monitoring the algal production of complex polysaccharides

Kristina Noack,ab  Björn Eskofier,c  Johannes Kiefer,*bd  Christina Dilk,a  Georg Bilow,a  Matthias Schirmer,e  Rainer Buchholzbe  and  Alfred Leipertzab 
Author affiliations

* Corresponding authors

a Institute of Engineering Thermodynamics, University of Erlangen-Nuremberg, Am Weichselgarten 9, 91058 Erlangen, Germany

b Erlangen Graduate School in Advanced Optical Technologies (SAOT), University of Erlangen-Nuremberg, Paul-Gordan-Straße 6, 91052 Erlangen, Germany

c Pattern Recognition Lab, University of Erlangen-Nuremberg, Martensstraße 3, 91058 Erlangen, Germany

d School of Engineering, University of Aberdeen, Fraser Noble Building, Aberdeen AB24 3UE, UK
E-mail: j.kiefer@abdn.ac.uk
Fax: +44 (0)1224-272497
Tel: +44 (0)1224-27-2495

e Lehrstuhl für Bioverfahrenstechnik, University of Erlangen-Nuremberg, Paul-Gordan-Straße 3, 91052 Erlangen, Germany

Abstract

The applicability of shifted-excitation Raman difference spectroscopy (SERDS) in combination with signal regression analysis as an alternative and non-invasive approach for monitoring the cultivation of phototrophic microorganisms producing complex molecules of pharmaceutical relevance in a bioreactor is demonstrated. As a model system, the cultivation of the red unicellular algae Porphyridium purpureum is used for focusing on the segregation of sulfated exopolysaccharides (EPS) which exhibit antiviral activity. The spectroscopic results obtained by partial linear least squares regression (PLSR) and by nonlinear support vector regression (SVR) are discussed against the corresponding results from the reference analytics based on the phenol–sulfuric acid assay. The SERDS-approach turns out to have strong potential as a non-invasive tool for online-monitoring of biotechnological processes.

Graphical abstract: Combined shifted-excitation Raman difference spectroscopy and support vector regression for monitoring the algal production of complex polysaccharides

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Publication details

The article was received on 10 Jun 2013, accepted on 16 Jul 2013 and first published on 17 Jul 2013


Article type: Paper
DOI: 10.1039/C3AN01158E
Citation: Analyst, 2013,138, 5639-5646
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    Combined shifted-excitation Raman difference spectroscopy and support vector regression for monitoring the algal production of complex polysaccharides

    K. Noack, B. Eskofier, J. Kiefer, C. Dilk, G. Bilow, M. Schirmer, R. Buchholz and A. Leipertz, Analyst, 2013, 138, 5639
    DOI: 10.1039/C3AN01158E

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