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Issue 15, 2013
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Cerium oxide-triggered ‘one-to-many’ catalytic cycling strategy for in situ amplified electronic signal of low-abundance protein

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Abstract

Multifunctionalized thionine-modified cerium oxide (Thi–CeO2) nanostructures with redox ability and catalytic activity were designed as the bionanolabels for in situ amplified electronic signal of low-abundance protein (carcinoembryonic antigen, CEA, used as a model) based on a cerium oxide-triggered ‘one-to-many’ catalytic cycling strategy. Initially, the carried CeO2 nanoparticles autocatalytically hydrolyzed the phosphate ester bond of L-ascorbic acid 2-phosphate (AAP) to produce a new reactant (L-ascorbic acid, AA), then the generated AA was electrochemically oxidized by the assembled thionine on the Thi–CeO2, and the resultant product was then reduced back to AA by the added tris(2-carboxyethy)phosphine (TCEP). The catalytic cycling could be re-triggered by the thionine and TCEP, resulting in amplification of the electrochemical signal. Under the optimized conditions, the electrochemical immunosensor exhibited a wide linear range of 0.1 pg mL−1 to 80 ng mL−1 with a low detection limit of 0.08 pg mL−1 CEA at the 3σblank level. In addition, the methodology was evaluated for the analysis of clinical serum samples, and was in good accordance with values obtained using the commercialized enzyme-linked immunosorbent assay (ELISA) method.

Graphical abstract: Cerium oxide-triggered ‘one-to-many’ catalytic cycling strategy for in situ amplified electronic signal of low-abundance protein

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Publication details

The article was received on 09 Apr 2013, accepted on 10 May 2013 and first published on 13 May 2013


Article type: Paper
DOI: 10.1039/C3AN00706E
Citation: Analyst, 2013,138, 4327-4333
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    Cerium oxide-triggered ‘one-to-many’ catalytic cycling strategy for in situ amplified electronic signal of low-abundance protein

    J. Tang, X. Chen, J. Zhou, Q. Li, G. Chen and D. Tang, Analyst, 2013, 138, 4327
    DOI: 10.1039/C3AN00706E

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