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Issue 14, 2013
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Exploring the spectroscopic differences of Caki-2 cells progressing through the cell cycle while proliferating in vitro

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Abstract

FTIR micro-spectral images of Caki-2 cells cytospun onto calcium fluoride (CaF2) slides were used to build a computational model in order to discriminate between the biochemical events of the continuous cell cycle during proliferation. Multivariate analysis and machine learning techniques such as PCA, PLSR and SVMs were used to highlight the chemical differences among the cell cycle phases and also to point out the need for removing the distortion of the spectra due to the morphology of the cells. Results showed cell cycle dependant scattering profiles that enabled the training of a SVM in order to recognise, with a relative high accuracy, each cell cycle phase purely with the scattering curve removed from the FTIR data after being subject to the RMieS-EMSC algorithm.

Graphical abstract: Exploring the spectroscopic differences of Caki-2 cells progressing through the cell cycle while proliferating in vitro

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Publication details

The article was received on 13 Mar 2013, accepted on 23 Apr 2013 and first published on 24 Apr 2013


Article type: Paper
DOI: 10.1039/C3AN00507K
Citation: Analyst, 2013,138, 3957-3966
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    Exploring the spectroscopic differences of Caki-2 cells progressing through the cell cycle while proliferating in vitro

    M. Jimenez-Hernandez, C. Hughes, P. Bassan, F. Ball, M. D. Brown, N. W. Clarke and P. Gardner, Analyst, 2013, 138, 3957
    DOI: 10.1039/C3AN00507K

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