Jump to main content
Jump to site search

Issue 14, 2013
Previous Article Next Article

Substrate contributions in micro-ATR of thin samples: implications for analysis of cells, tissue and biological fluids

Author affiliations

Abstract

Low-e microscope slides are a common substrate for biological samples. Typically they are used for transflection infrared microspectroscopy but increasingly they are also being used for micro-ATR experiments since it is assumed that the FTIR-ATR absorbance spectra of cells and tissue on low-e substrates will not contain any spectral contributions from the substrate materials. This, in part, is due to the expectation that all the infrared light will be reflected at the highly reflective surface. At low sample thicknesses, however (e.g. less than 2 μm) the electric field does indeed penetrate through the substrate layers and undergoes absorption, from the glass supporting layer making up the majority of the slide. In this paper we show experimental evidence of the substrate contributions in ATR spectra and also a theoretical model giving insight into the spectral contributions of the substrate as a function of sample thickness.

Graphical abstract: Substrate contributions in micro-ATR of thin samples: implications for analysis of cells, tissue and biological fluids

Back to tab navigation

Publication details

The article was received on 20 Feb 2013, accepted on 31 May 2013 and first published on 31 May 2013


Article type: Paper
DOI: 10.1039/C3AN00363A
Citation: Analyst, 2013,138, 4139-4146
  •   Request permissions

    Substrate contributions in micro-ATR of thin samples: implications for analysis of cells, tissue and biological fluids

    P. Bassan, A. Sachdeva, J. Lee and P. Gardner, Analyst, 2013, 138, 4139
    DOI: 10.1039/C3AN00363A

Search articles by author

Spotlight

Advertisements