Jump to main content
Jump to site search

Issue 11, 2013
Previous Article Next Article

A polymeric waveguide resonant mirror (RM) device for detection in microfluidic flow cells

Author affiliations

Abstract

A novel resonant mirror (RM) device, which consisted of silica sol–gel spacer and polystyrene waveguide layers on a standard microscope slide glass substrate, was developed in this work. The device was successfully used to measure the absorption spectrum of methylene blue with a limit of detection (LOD) of at most 20.8 μM at 635 nm and a minimum detectable absorption coefficient of 0.94 cm−1. A RM device consisting of dye-doped polystyrene waveguide layer was then demonstrated to be suitable to monitor antibody–antigen (in this case, anti-IgG and IgG) binding and was shown to be capable of detecting at least 100 nM IgG. The sensitivity of the device was estimated to be 17.27° per refractive index unit (RIU), which corresponds to a resolution of 1.45 × 10−4 RIU for the set-up used. The RM device developed in this work can be easily integrated with microfluidic devices to identify and quantify (bio) chemical species by either absorption spectroscopy or measurement of effective optical thickness or both. In addition, the device was fabricated using a simple and low cost fabrication technique, spin coating. Hence, it can be easily mass produced.

Graphical abstract: A polymeric waveguide resonant mirror (RM) device for detection in microfluidic flow cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 02 Feb 2013, accepted on 04 Apr 2013 and first published on 05 Apr 2013


Article type: Paper
DOI: 10.1039/C3AN00263B
Citation: Analyst, 2013,138, 3209-3215
  •   Request permissions

    A polymeric waveguide resonant mirror (RM) device for detection in microfluidic flow cells

    R. Gupta and N. J. Goddard, Analyst, 2013, 138, 3209
    DOI: 10.1039/C3AN00263B

Search articles by author

Spotlight

Advertisements