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Issue 13, 2013
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An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification

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Abstract

To develop a portable point-of-care system based on biosensors for common infectious diseases such as urinary tract infection, the sensing process needs to be implemented within an enclosed fluidic system. On chip sample preparation of clinical samples remains a significant obstacle to achieving robust sensor performance. Herein AC electrokinetics is applied in an electrochemical biosensor cassette to enhance molecular convection and hybridization efficiency through electrokinetics induced fluid motion and Joule heating induced temperature elevation. Using E. coli as an exemplary pathogen, we determined the optimal electrokinetic parameters for detecting bacterial 16S rRNA in the biosensor cassette based on the current output, signal-to-noise ratio, and limit of detection. In addition, a panel of six probe sets targeting common uropathogenic bacteria was demonstrated. The optimized parameters were also validated using patient-derived clinical urine samples. The effectiveness of electrokinetics for on chip sample preparation will facilitate the implementation of point-of-care diagnosis of urinary tract infection in the future.

Graphical abstract: An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification

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Publication details

The article was received on 01 Feb 2013, accepted on 06 Apr 2013 and first published on 24 Apr 2013


Article type: Paper
DOI: 10.1039/C3AN00259D
Citation: Analyst, 2013,138, 3660-3666
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    An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification

    M. Ouyang, R. Mohan, Y. Lu, T. Liu, K. E. Mach, M. L. Y. Sin, M. McComb, J. Joshi, V. Gau, P. K. Wong and J. C. Liao, Analyst, 2013, 138, 3660
    DOI: 10.1039/C3AN00259D

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