Fast and selective recognizes polysaccharide by surface molecularly imprinted film coated onto aldehyde-modified magnetic nanoparticles

Abstract

In this work, a starch imprinted magnetic nanoparticles composite material has been successfully synthesized. This molecular imprinted material has promising practical utility in capturing polysaccharides for pharmacology applications. First, we synthesized Fe₃O₄ nanoparticles by coprecipitation, followed by the modification of tetraethyl orthosilicate (TEOS) and functional amino group and aldehyde group, respectively. Then we used functionalized Fe₃O₄@SiO₂ as the magnetic cores, starch as the template, 3-aminophenylboronic acid (APBA) as the functional monomer and ammonium persulphate (APS) as the initiator. Magnetic molecularly imprinted nanoparticles (MMIPs) were synthesized by surface-imprinted polymerization under airtight tubes at room temperature for 24 h. MMIPs were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM) analysis. This showed a high saturation magnetization value (5.59 emu g⁻¹) easily reached under an external magnetic field. The binding experiments were shown to have relatively high adsorption capacity (15.45 mg g⁻¹) and selective recognition ability over structurally related compounds. Therefore, MMIPs provide a sensitive and selective approach and offer the potential to become a new key for polysaccharide separation and purification.