Jump to main content
Jump to site search

Issue 1, 2013
Previous Article Next Article

Integration of rapid DNA hybridization and capillary zone electrophoresis using bidirectional isotachophoresis

Author affiliations

Abstract

We present a method for rapid, sequence-specific detection of multiple DNA fragments by integrating isotachophoresis (ITP) based DNA hybridization and capillary zone electrophoresis (CZE) using bidirectional ITP. Our method leverages the high preconcentration ability of ITP to accelerate slow, second-order DNA hybridization kinetics, and the high resolving power of CZE to separate and identify reaction products. We demonstrate the speed and sensitivity of our assay by detecting 5 pM, 39 nt ssDNA target within 3 min, using a molecular beacon probe. We also demonstrate the feasibility of our assay for multiplexed detection of multiple-length ssDNA targets by simultaneously detecting 39 and 90 nt ssDNA targets.

Graphical abstract: Integration of rapid DNA hybridization and capillary zone electrophoresis using bidirectional isotachophoresis

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Sep 2012, accepted on 13 Oct 2012 and first published on 15 Oct 2012


Article type: Communication
DOI: 10.1039/C2AN36249J
Citation: Analyst, 2013,138, 87-90
  •   Request permissions

    Integration of rapid DNA hybridization and capillary zone electrophoresis using bidirectional isotachophoresis

    S. S. Bahga, C. M. Han and J. G. Santiago, Analyst, 2013, 138, 87
    DOI: 10.1039/C2AN36249J

Search articles by author

Spotlight

Advertisements