Microrheology uses the motion of dispersed colloidal probe particles to measure the viscosity or viscoelastic moduli of soft materials. The distinct advantages of microrheology include small sample volume requirements, access to a large range of time scales for the dynamic response and short acquisition times. These advantages make microrheology important for studies of biomaterial hydrogelators. Recent advances have enabled the precise characterization of hydrogelator sol–gel transitions, measurements of rare and scarce materials and high-throughput screening of hydrogel rheology over a large composition space. In this review, we focus on multiple particle tracking microrheology, including the considerations that define its operating regimes and its recent applications. Those interested in biomaterial rheology will find these methods as accessible as bulk rheological measurements and straightforward to implement in their own work.