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Issue 6, 2012
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Engineering a gold-specific regulon for cell-based visual detection and recovery of gold

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Abstract

A gold-specific sensory protein GolS from Samonella gol regulon was incorporated into E. coli, which in conjunction with an engineered downstream red fluorescence protein allowed the highly sensitive and selective whole-cell detection of gold(III) ions by naked eyes. The putative gold-chaperone, GolB, in the gol regulon was next verified to be specific to gold(I) ions over other metal ions including copper(I). The subsequent display of GolB on E. coli cell surface permitted selective enrichment of gold ions from media containing various thiophilic metal ions. The cell surface-enriched gold(I) was further shown to be easily recovered and the gold-deprived bacteria were capable for re-usage. E. coli bacteria harboring these gold-specific elements from the gol regulon could be a valuable tool for visual detection and facile recycling of gold ions from environmental resources.

Graphical abstract: Engineering a gold-specific regulon for cell-based visual detection and recovery of gold

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Publication details

The article was received on 25 Dec 2011, accepted on 08 Mar 2012 and first published on 09 Mar 2012


Article type: Edge Article
DOI: 10.1039/C2SC01119K
Citation: Chem. Sci., 2012,3, 1780-1784
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    Engineering a gold-specific regulon for cell-based visual detection and recovery of gold

    W. Wei, T. Zhu, Y. Wang, H. Yang, Z. Hao, P. R. Chen and J. Zhao, Chem. Sci., 2012, 3, 1780
    DOI: 10.1039/C2SC01119K

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