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Issue 3, 2012
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Dip-pen nanolithography of optically transparent cationic polymers to manipulate spatial organization of proteolipid membranes

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Abstract

Herein we report on an approach to use dip-pen nanolithography (DPN) to rapidly prototype nano- and microscale cationic polymer structures that guide the transport of lipid molecules in a fluid membrane. An atomic force microscope (AFM) tip is used to transfer poly(diallyldimethylammonium chloride) (PDAC) to a silica surface that templates the self-assembly of a phospholipid bilayer. Based on AFM and high-resolution optical microscopy data, DPN-generated PDAC structures are optically transparent and composed of up to three molecular layers of polyelectrolyte. We demonstrate that patterns can juxtapose mobile and immobile ligands in a supported lipid bilayer that simultaneously engages the epidermal growth factor receptor (EGFR) and adhesion receptors on the cell surface. In principle, DPN-generated polyelectrolyte structures have important applications in investigating supramolecular protein assemblies in living cells.

Graphical abstract: Dip-pen nanolithography of optically transparent cationic polymers to manipulate spatial organization of proteolipid membranes

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Publication details

The article was received on 17 Jul 2011, accepted on 16 Nov 2011 and first published on 01 Dec 2011


Article type: Edge Article
DOI: 10.1039/C1SC00475A
Citation: Chem. Sci., 2012,3, 794-799
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    Dip-pen nanolithography of optically transparent cationic polymers to manipulate spatial organization of proteolipid membranes

    Y. Narui and K. S. Salaita, Chem. Sci., 2012, 3, 794
    DOI: 10.1039/C1SC00475A

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