Jump to main content
Jump to site search

Issue 7, 2012
Previous Article Next Article

Synthetic 5′-phosphorylated oligodeoxynucleotide purification through catching full-length sequences by polymerization

Author affiliations

Abstract

The readily scalable catching by polymerization purification technology has been further advanced to purify 5′-phosphorylated synthetic oligodeoxynucleotides (ODNs). The new technology utilizes a phosphoramidite that contains a fluoride-cleavable diisopropylsilyl acetal linker and a polymerizable methacrylamide group, and is capable of phosphorylation of ODN. For purification, the phosphoramidite was coupled to the 5′-end of full-length ODN on a synthesizer. Because failure sequences were capped in each synthetic cycle, only the full-length sequences were phosphinylated and acrylated. After cleavage and deprotection, the crude ODN was subjected to polymerization under typical acrylamide gel formation conditions. The full-length ODN was incorporated into polymer. The failure sequences and other impurities were simply removed by washing with water. Pure full-length ODN that contained a 5′-phosphate group was cleaved from the polymer with HF–pyridine. Reversed-phase (RP) HPLC showed that the ODN was pure, and the recovery yield was higher than that of typical preparative HPLC purification.

Graphical abstract: Synthetic 5′-phosphorylated oligodeoxynucleotide purification through catching full-length sequences by polymerization

Back to tab navigation

Supplementary files

Publication details

The article was received on 25 Dec 2011, accepted on 04 Jan 2012 and first published on 05 Jan 2012


Article type: Paper
DOI: 10.1039/C2RA01357F
Citation: RSC Adv., 2012,2, 2803-2808
  •   Request permissions

    Synthetic 5′-phosphorylated oligodeoxynucleotide purification through catching full-length sequences by polymerization

    Y. Yuan, S. Fueangfung, X. Lin, D. Pokharel and S. Fang, RSC Adv., 2012, 2, 2803
    DOI: 10.1039/C2RA01357F

Search articles by author

Spotlight

Advertisements