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Issue 6, 2012
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The development of affinity capture devices—a nanoscale purification platform for biological in situtransmission electron microscopy

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Abstract

The use of in situtransmission electron microscopy (TEM) to study biological processes is a new frontier in high-resolution imaging. Here we report the development of affinity capture devices that are used to isolate biological entities for in situ studies. Affinity capture devices are silicon nitride microchips coated with functionalized lipid monolayers. We tested the isolation capacity of the devices by using protein synthesis machinery as a test specimen for single particle and in situTEM analysis. Overall, we demonstrate the feasibility of purifying biological assemblies in a liquid environment within a TEM column. This novel application may serve to bridge the gap between cellular and molecular imaging techniques.

Graphical abstract: The development of affinity capture devices—a nanoscale purification platform for biological in situtransmission electron microscopy

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Publication details

The article was received on 22 Nov 2011, accepted on 12 Dec 2011 and first published on 01 Feb 2012


Article type: Paper
DOI: 10.1039/C2RA01163H
Citation: RSC Adv., 2012,2, 2408-2412
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    The development of affinity capture devices—a nanoscale purification platform for biological in situtransmission electron microscopy

    K. Degen, M. Dukes, J. R. Tanner and D. F. Kelly, RSC Adv., 2012, 2, 2408
    DOI: 10.1039/C2RA01163H

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