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Issue 11, 2012
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Electronically excited states of DNA oligonucleotides with disordered base sequences studied by fluorescence spectroscopy

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Abstract

DNA double-stranded oligomers are studied by steady-state and time-resolved fluorescence spectroscopy from the femtosecond to the nanosecond time-scale, following excitation at 267 nm. It is shown that emission arises from three types of excited states. (i) Bright ππ* states emitting around 330 nm and decaying on the sub-picosecond time-scale with an average lifetime of ca. 0.4 ps and a quantum yield lower than 4 × 10−6. (ii) Excimers/exciplexes emitting around 430 nm and decaying on the sub-nanosecond time-scale. (iii) Excited states emitting mainly at short wavelengths (λ < 330 nm) and decaying on the nanosecond time-scale, possibly correlated to GC pairs. The properties of the examined duplexes, exhibiting significant disorder with respect to the nearest neighbour base sequence, are radically different than those of the much longer and disordered calf thymus DNA. Such behaviour suggests that long range and/or sequence effects play a key role in the fate of excitation energy.

Graphical abstract: Electronically excited states of DNA oligonucleotides with disordered base sequences studied by fluorescence spectroscopy

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Publication details

The article was received on 09 Jun 2012, accepted on 18 Sep 2012 and first published on 19 Sep 2012


Article type: Paper
DOI: 10.1039/C2PP25180A
Citation: Photochem. Photobiol. Sci., 2012,11, 1767-1773
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    Electronically excited states of DNA oligonucleotides with disordered base sequences studied by fluorescence spectroscopy

    I. Vayá, J. Brazard, T. Gustavsson and D. Markovitsi, Photochem. Photobiol. Sci., 2012, 11, 1767
    DOI: 10.1039/C2PP25180A

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