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Issue 7, 2012
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Stem cell-based photodynamic therapy

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Abstract

We have transfected murine neural stem cells (NSCs) and rat umbilical cord matrix-derived stem cells (RUCMSCs) with a plasmid expressing gaussia luciferase (gLuc). These cells are engineered to secrete the luciferase. We have used gLuc containing supernatant from culturing the NSCs to perform in vitro photodynamic therapy of murine melanoma cells (B16F10), and RUCMSCs to perform in vivo PDT of lung melanomas in C57BL/6 mice. The treatment system was comprised of aminolevulic acid as a prodrug for the synthesis of the photosensitizer protoporphyrin IX, gaussia luciferase, and its’ substrate coelenterazine. A significant reduction of the number of live melanoma cells in vitro and a borderline significant retardation of tumour growth in vivo was observed after coelenterazine-mediated PDT.

Graphical abstract: Stem cell-based photodynamic therapy

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Publication details

The article was received on 31 Dec 2011, accepted on 10 Apr 2012 and first published on 08 May 2012


Article type: Paper
DOI: 10.1039/C2PP05417E
Citation: Photochem. Photobiol. Sci., 2012,11, 1251-1258
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    Stem cell-based photodynamic therapy

    T. B. Shrestha, G. M. Seo, M. T. Basel, M. Kalita, H. Wang, D. Villanueva, M. Pyle, S. Balivada, R. S. Rachakatla, H. Shinogle, P. S. Thapa, D. Moore, D. L. Troyer and S. H. Bossmann, Photochem. Photobiol. Sci., 2012, 11, 1251
    DOI: 10.1039/C2PP05417E

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