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Issue 3, 2012
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Externally sensitized deprotection of PPG-masked carbonyls as a spatial proximity probe in photoamplified detection of binding events

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Abstract

Externally sensitized electron-transfer fragmentation in dithiane PPG (photoremovable protecting group)-protected carbonyls is adopted for detection and amplification of molecular recognition events. The new methodology allows for detection of as low as 50 attomoles of avidin utilizing an imager based on a low sensitivity mass-produced consumer CCD camera. Numeric modelling is carried out to demonstrate the intrinsic limitations of 2D amplification on surfaces and the advantages of unconstrained amplification in a compartmentalized volume of spatially addressable 3D solutions.

Graphical abstract: Externally sensitized deprotection of PPG-masked carbonyls as a spatial proximity probe in photoamplified detection of binding events

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Publication details

The article was received on 01 Oct 2011, accepted on 15 Dec 2011 and first published on 18 Jan 2012


Article type: Paper
DOI: 10.1039/C2PP05326H
Citation: Photochem. Photobiol. Sci., 2012,11, 564-577
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    Externally sensitized deprotection of PPG-masked carbonyls as a spatial proximity probe in photoamplified detection of binding events

    T. P. Gustafson, G. A. Metzel and A. G. Kutateladze, Photochem. Photobiol. Sci., 2012, 11, 564
    DOI: 10.1039/C2PP05326H

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