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Issue 4, 2012
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The effect of hydration on the UV absorption coefficient of intact melanosomes

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Abstract

The physical properties of melanosomes have been shown to depend on water content. Herein, the ultraviolet absorption coefficient at λ = 244 nm for intact bovine choroidal melanosomes is determined from photoemission electron microscopy images recorded as a function of vacuum exposure. The dehydration of the melanosome under ultra-high vacuum manifests itself by a decrease in the absorption coefficient to about 60% of its initial value, and a concomitant increase in its image brightness. This change in the absorption of the melanosome is consistent with the influence of solvent polarity on the UV absorption coefficient of model systems for the pigment eumelanin, the predominant UV absorber contained in the choroid melanosomes.

Graphical abstract: The effect of hydration on the UV absorption coefficient of intact melanosomes

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Publication details

The article was received on 26 Jul 2011, accepted on 21 Nov 2011 and first published on 09 Jan 2012


Article type: Paper
DOI: 10.1039/C2PP05233D
Citation: Photochem. Photobiol. Sci., 2012,11, 687-691
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    The effect of hydration on the UV absorption coefficient of intact melanosomes

    E. Lin, D. N. Peles and J. D. Simon, Photochem. Photobiol. Sci., 2012, 11, 687
    DOI: 10.1039/C2PP05233D

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