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Issue 40, 2012
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FRET-based ratiometric detection of Hg2+ and biothiols using naphthalimide–rhodamine dyads

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Abstract

A new naphthalimide–rhodamine-based dyad 1 in CH3CN–HEPES (1 : 1) buffer solution exhibits fluorescence resonance energy transfer (FRET) from naphthalimide to the rhodamine moiety on addition of only Hg2+ ions and allows ratiometric absorption and fluorimetric estimation of Hg2+ ions between 50 nM (10 ppb) to 2 μM (0.4 ppm). FRET-induced fluorescence changes were recovered again by the subsequent addition of thiol amino acids via reverse FRET. The interconversion of probe 1 and 1·Hg2+2+2+via the complexation/decomplexation by the modulation of Hg2+/Cys exhibited a selective probe for biothiols in real samples.

Graphical abstract: FRET-based ratiometric detection of Hg2+ and biothiols using naphthalimide–rhodamine dyads

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Publication details

The article was received on 25 Apr 2012, accepted on 08 Aug 2012 and first published on 08 Aug 2012


Article type: Paper
DOI: 10.1039/C2OB25794G
Citation: Org. Biomol. Chem., 2012,10, 8076-8081
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    FRET-based ratiometric detection of Hg2+ and biothiols using naphthalimide–rhodamine dyads

    V. Luxami, M. Verma, R. Rani, K. Paul and S. Kumar, Org. Biomol. Chem., 2012, 10, 8076
    DOI: 10.1039/C2OB25794G

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