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Issue 31, 2012
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From pico to nano: biofunctionalization of cube-octameric silsesquioxanes by peptides and miniproteins

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Abstract

Polyhedral silsesquioxanes are considered valuable conjugation scaffolds. Nevertheless, only a few examples of silsesquioxane-assembled peptide oligomers have been reported to date. We developed a new bioorthogonal cube-octameric silsesquioxane (COSS) scaffold bearing eight aminooxy coupling sites allowing for the conjugation of diverse peptides via oxime ligation. We found that the coupling efficacy depends on the ligand in view of steric hindrance and electrostatic repulsion. For the first time scaffold-based conjugation of cystine-knot miniproteins having a backbone of about thirty amino acids was successfully accomplished without loss of bioactivity. Atomic force microscopy (AFM) provided further knowledge on the size of COSS verifying them as picoscaffolds growing upon bioconjugation to nano-dimension.

Graphical abstract: From pico to nano: biofunctionalization of cube-octameric silsesquioxanes by peptides and miniproteins

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Publication details

The article was received on 13 Apr 2012, accepted on 11 Jun 2012 and first published on 13 Jun 2012


Article type: Paper
DOI: 10.1039/C2OB25728A
Citation: Org. Biomol. Chem., 2012,10, 6287-6293
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    From pico to nano: biofunctionalization of cube-octameric silsesquioxanes by peptides and miniproteins

    S. Fabritz, S. Hörner, D. Könning, M. Empting, M. Reinwarth, C. Dietz, B. Glotzbach, H. Frauendorf, H. Kolmar and O. Avrutina, Org. Biomol. Chem., 2012, 10, 6287
    DOI: 10.1039/C2OB25728A

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