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Two fluorescent lysine amide analogs, in which the carboxyl end of lysine was covalently attached to dansyl or NBD groups through an ethylene glycol-based linker, were rationally designed and synthesized. Both probes showed high binding affinity to the lysine riboswitch in vitro and their fluorescence intensities decreased by riboswitch binding.
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Organic & Biomolecular Chemistry
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