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A new naphthalimide–rhodamine-based dyad 1 in CH3CN–HEPES (1:1) buffer solution exhibits fluorescence resonance energy transfer (FRET) from naphthalimide to the rhodamine moiety on addition of only Hg2+ ions and allows ratiometric absorption and fluorimetric estimation of Hg2+ ions between 50 nM (10 ppb) to 2 μM (0.4 ppm). FRET-induced fluorescence changes were recovered again by the subsequent addition of thiol amino acids via reverse FRET. The interconversion of probe 1 and 1·Hg2+via the complexation/decomplexation by the modulation of Hg2+/Cys exhibited a selective probe for biothiols in real samples.
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Organic & Biomolecular Chemistry
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