FRET-based ratiometric detection of Hg2+ and biothiols using naphthalimide–rhodamine dyads

Vijay Luxami; Meenakshi Verma; Richa Rani; Kamaldeep Paul; Subodh Kumar

doi:10.1039/C2OB25794G

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Organic & Biomolecular Chemistry

Issue 40, 2012

The international home of synthetic, physical and biomolecular organic chemistry.

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Paper

FRET-based ratiometric detection of Hg²⁺ and biothiols using naphthalimide–rhodamine dyads

Vijay Luxami,*^a Meenakshi Verma,^a Richa Rani,^a Kamaldeep Paul^a and Subodh Kumar^b

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School of Chemistry and Biochemistry, Thapar University, Patiala-147004, India
E-mail: vj_luxami@yahoo.co.in

Department of Chemistry, UGC Center for Advanced Studies, Guru Nanak Dev University, Amritsar – 143 005, India

Org. Biomol. Chem., 2012,10, 8076-8081

DOI: 10.1039/C2OB25794G
Received 25 Apr 2012, Accepted 08 Aug 2012
First published online 08 Aug 2012

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Abstract
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A new naphthalimide–rhodamine-based dyad 1 in CH₃CN–HEPES (1:1) buffer solution exhibits fluorescence resonance energy transfer (FRET) from naphthalimide to the rhodamine moiety on addition of only Hg²⁺ ions and allows ratiometric absorption and fluorimetric estimation of Hg²⁺ ions between 50 nM (10 ppb) to 2 μM (0.4 ppm). FRET-induced fluorescence changes were recovered again by the subsequent addition of thiol amino acids via reverse FRET. The interconversion of probe 1 and 1·Hg²⁺via the complexation/decomplexation by the modulation of Hg²⁺/Cys exhibited a selective probe for biothiols in real samples.