Issue 3, 2012

Click-enabled heterotrifunctional template for sequential bioconjugations

Abstract

A heterotrifunctional template was developed that utilizes thiolmaleimide and click chemistries (both copper-free and copper-mediated) to effect sequential biomolecule conjugations in a one-pot process. The breadth of compatible substrates was illustrated through highly efficient conjugations of protein, peptide, sugar, lipid, fluoroalkane, biotin and fluorophore molecules. This template should be useful for the creation of chemically-enhanced/enabled biotherapeutics, especially through the expression of discontinuous (and heterogeneous) epitopes.

Graphical abstract: Click-enabled heterotrifunctional template for sequential bioconjugations

Supplementary files

Article information

Article type
Paper
Submitted
16 Aug 2011
Accepted
10 Oct 2011
First published
10 Oct 2011

Org. Biomol. Chem., 2012,10, 548-554

Click-enabled heterotrifunctional template for sequential bioconjugations

D. M. Beal, V. E. Albrow, G. Burslem, L. Hitchen, C. Fernandes, C. Lapthorn, L. R. Roberts, M. D. Selby and L. H. Jones, Org. Biomol. Chem., 2012, 10, 548 DOI: 10.1039/C1OB06398G

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