Jump to main content
Jump to site search

Issue 1, 2012
Previous Article Next Article

Synthesis of nucleoside mono- and triphosphates bearing oligopyridine ligands, their incorporation into DNA and complexation with transition metals

Author affiliations

Abstract

Modified nucleoside mono- (dARMPs and dCRMPs) and triphosphates (dARTPs and dCRTPs) bearing bipyridine or terpyridine ligands attached viaacetylene linker were prepared by single-step aqueous-phase Sonogashira cross-coupling of 7-iodo-7-deaza-dAMP or -dATP, and 5-iodo-dCMP or -dCTP with the corresponding bipyridine- or terpyridine-linked acetylenes. The modified dNRTPs were successfully incorporated into the oligonucleotides by primer extension experiment (PEX) using different DNA polymerases and the PEX products were used for post-synthetic complexation with Fe2+.

Graphical abstract: Synthesis of nucleoside mono- and triphosphates bearing oligopyridine ligands, their incorporation into DNA and complexation with transition metals

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 Aug 2011, accepted on 27 Sep 2011 and first published on 27 Sep 2011


Article type: Paper
DOI: 10.1039/C1OB06359F
Citation: Org. Biomol. Chem., 2012,10, 49-55
  •   Request permissions

    Synthesis of nucleoside mono- and triphosphates bearing oligopyridine ligands, their incorporation into DNA and complexation with transition metals

    L. Kalachova, R. Pohl and M. Hocek, Org. Biomol. Chem., 2012, 10, 49
    DOI: 10.1039/C1OB06359F

Search articles by author

Spotlight

Advertisements