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Issue 12, 2012
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Guiding plant virus particles to integrin-displaying cells

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Abstract

Viral nanoparticles (VNPs) are structurally regular, highly stable, tunable nanomaterials that can be conveniently produced in high yields. Unmodified VNPs from plants and bacteria generally do not show tissue specificity or high selectivity in binding to or entry into mammalian cells. They are, however, malleable by both genetic and chemical means, making them useful scaffolds for the display of large numbers of cell- and tissue-targeting ligands, imaging moieties, and/or therapeutic agents in a well-defined manner. Capitalizing on this attribute, we modified the genetic sequence of the Cowpea mosaic virus (CPMV) coat protein to display an RGD oligopeptide sequence derived from human adenovirus type 2 (HAdV-2). Concurrently, wild-type CPMV was modified via NHS acylation and Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC) chemistry to attach an integrin-binding cyclic RGD peptide. Both types of particles showed strong and selective affinity for several different cancer cell lines that express RGD-binding integrin receptors.

Graphical abstract: Guiding plant virus particles to integrin-displaying cells

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Publication details

The article was received on 09 Mar 2012, accepted on 10 Apr 2012 and first published on 15 May 2012


Article type: Paper
DOI: 10.1039/C2NR30571B
Citation: Nanoscale, 2012,4, 3698-3705
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    Guiding plant virus particles to integrin-displaying cells

    M. L. Hovlid, N. F. Steinmetz, B. Laufer, J. L. Lau, J. Kuzelka, Q. Wang, T. Hyypiä, G. R. Nemerow, H. Kessler, M. Manchester and M. G. Finn, Nanoscale, 2012, 4, 3698
    DOI: 10.1039/C2NR30571B

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