Bio-functionalization of ligand-free upconverting lanthanide doped nanoparticles for bio-imaging and cell targeting

Nicoleta Bogdan; Emma Martín Rodríguez; Francisco Sanz-Rodríguez; Ma Carmen Iglesias de la Cruz; Ángeles Juarranz; Daniel Jaque; José García Solé; John A. Capobianco

doi:10.1039/C2NR30982C

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Nanoscale

Issue 12, 2012

A new peer reviewed journal publishing experimental and theoretical work across the breadth of nanoscience and nanotechnology

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Bio-functionalization of ligand-free upconverting lanthanide doped nanoparticles for bio-imaging and cell targeting

Nicoleta Bogdan,^a Emma Martín Rodríguez,^a Francisco Sanz-Rodríguez,^b M<small xmlns="http://www.rsc.org/schema/rscart38"> <sup>a</sup> </small> Carmen Iglesias de la Cruz,^c Ángeles Juarranz,^b Daniel Jaque,^d José García Solé^d and John A. Capobianco*^a

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Department of Chemistry and Biochemistry and Centre for Research in NanoScience, Concordia University, 7141 Sherbrooke Street West, Montreal, Canada
E-mail: capo@vax2.concordia.ca

Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain

Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, 28049 Madrid, Spain

Departamento de Física de Materiales, C-IV, Universidad Autónoma de Madrid, C/Francisco Tomas y Valiente 7, 28049 Madrid, Spain

Nanoscale, 2012,4, 3647-3650

DOI: 10.1039/C2NR30982C
Received 24 Apr 2012, Accepted 14 May 2012
First published online 15 May 2012

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We report on the functionalization of ligand-free NaGdF₄:Er³⁺, Yb³⁺ upconverting nanoparticles with heparin and basic fibroblast growth factor (bFGF). These upconverting nanoparticles are used to obtain high-contrast images of HeLa cells. These images reveal that the heparin–bFGF functionalized nanoparticles show specific binding to the cell membrane.

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Nicoleta Bogdan
Emma Martín Rodríguez
Francisco Sanz-Rodríguez
Ma Carmen Iglesias de la Cruz
Ángeles Juarranz
Daniel Jaque
José García Solé
John A. Capobianco

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Nanoscale

Bio-functionalization of ligand-free upconverting lanthanide doped nanoparticles for bio-imaging and cell targeting

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