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Issue 12, 2012
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Concentration of various trace elements in the rat retina and their distribution in different structures

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Abstract

Inductively coupled plasma mass spectrometry (ICP-MS) was used to quantify the total amount of trace elements in retina from adult male Sprague-Dawley rats (n = 6). Concentration of trace elements within individual retinal areas in frozen sections of the fellow eye was established with the use of two methodologies: (1) particle-induced X-ray emission (PIXE) in combination with 3D depth profiling with Rutherford backscattering spectrometry (RBS) and (2) synchrotron X-ray fluorescence (SXRF) microscopy. The most abundant metal in the retina was zinc, followed by iron and copper. Nickel, manganese, chromium, cobalt, selenium and cadmium were present in very small amounts. The PIXE and SXRF analysis yielded a non-homogenous pattern distribution of metals in the retina. Relatively high levels of zinc were found in the inner part of the photoreceptor inner segments (RIS)/outer limiting membrane (OLM), inner nuclear layer and plexiform layers. Iron was found to accumulate in the retinal pigment epithelium/choroid layer and RIS/OLM. Copper in turn, was localised primarily in the RIS/OLM and plexiform layers. The trace elements iron, copper, and zinc exist in different amounts and locations in the rat retina.

Graphical abstract: Concentration of various trace elements in the rat retina and their distribution in different structures

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Publication details

The article was received on 04 Aug 2012, accepted on 27 Sep 2012 and first published on 28 Sep 2012


Article type: Paper
DOI: 10.1039/C2MT20157G
Citation: Metallomics, 2012,4, 1245-1254
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    Concentration of various trace elements in the rat retina and their distribution in different structures

    M. Ugarte, G. W. Grime, G. Lord, K. Geraki, J. F. Collingwood, M. E. Finnegan, H. Farnfield, M. Merchant, M. J. Bailey, N. I. Ward, P. J. Foster, P. N. Bishop and N. N. Osborne, Metallomics, 2012, 4, 1245
    DOI: 10.1039/C2MT20157G

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