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Issue 8, 2012
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Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting proteinprotein interactions in vivo

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Abstract

Split-GFP reassembly is an operationally simple in vivo technique used to identify and study interactions involving proteins and/or peptides. However, the instability of split-GFP fragments and their susceptibility to aggregation place limitations on the broader use of split-GFP reassembly. Supercharged proteins, including supercharged GFP, are variants with high theoretical negative or positive charge that are resistant to aggregation. We show that a split-superpositive GFP (split-spGFP) variant reassembles faster and more efficiently than previously reported split-sg100 GFP and split-folding-reporter GFP (split-frGFP) systems. In addition, interaction-dependent split-spGFP reassembly is efficient at physiological temperature. The increased efficiency and robustness of split-spGFP reassembly make this reporter system ideal for identifying and studying interactions involving proteins and/or peptides in vivo, and may be particularly useful for identifying or studying interactions involving proteins or peptides that are themselves susceptible to aggregation.

Graphical abstract: Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting protein–protein interactions in vivo

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Publication details

The article was received on 02 Apr 2012, accepted on 22 May 2012 and first published on 24 May 2012


Article type: Method
DOI: 10.1039/C2MB25130B
Citation: Mol. BioSyst., 2012,8, 2036-2040
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    Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting proteinprotein interactions in vivo

    B. D. Blakeley, A. M. Chapman and B. R. McNaughton, Mol. BioSyst., 2012, 8, 2036
    DOI: 10.1039/C2MB25130B

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