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Issue 4, 2012
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Wide-range protein photo-crosslinking achieved by a genetically encoded Nε-(benzyloxycarbonyl)lysine derivative with a diazirinyl moiety

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Abstract

A derivative of Nε-benzyloxycarbonyl-L-lysine with a photo-reactive diazirinyl group, Nε-[((4-(3-(trifluoromethyl)-3H-diazirin-3-yl)benzyl)oxy)carbonyl]-L-lysine, was site-specifically incorporated into target proteins in mammalian cells. The incorporated photo-crosslinker is able to react not only with residues as distant as about 15 Å but also with those in closer proximity, thus enabling “wide-range” photo-crosslinking of proteins.

Graphical abstract: Wide-range protein photo-crosslinking achieved by a genetically encoded Nε-(benzyloxycarbonyl)lysine derivative with a diazirinyl moiety

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Publication details

The article was received on 02 Aug 2011, accepted on 12 Jan 2012 and first published on 01 Feb 2012


Article type: Communication
DOI: 10.1039/C2MB05321G
Citation: Mol. BioSyst., 2012,8, 1131-1135
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    Wide-range protein photo-crosslinking achieved by a genetically encoded Nε-(benzyloxycarbonyl)lysine derivative with a diazirinyl moiety

    T. Yanagisawa, N. Hino, F. Iraha, T. Mukai, K. Sakamoto and S. Yokoyama, Mol. BioSyst., 2012, 8, 1131
    DOI: 10.1039/C2MB05321G

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