Jump to main content
Jump to site search

Issue 7, 2012
Previous Article Next Article

Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling

Author affiliations

Abstract

The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps. In this study, the mobile MutS clamps were trapped on DNA in a functional state using single-cysteine variants of MutS and thiol-modified homoduplex or heteroduplex DNA. This approach allows stabilization of various transient MutS–DNA complexes and will enable their structural and functional analysis.

Graphical abstract: Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 12 Mar 2012, accepted on 16 Apr 2012 and first published on 17 Apr 2012


Article type: Communication
DOI: 10.1039/C2MB25086A
Citation: Mol. BioSyst., 2012,8, 1861-1864
  •   Request permissions

    Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling

    R. J. Heinze, S. Sekerina, I. Winkler, C. Biertümpfel, T. S. Oretskaya, E. Kubareva and P. Friedhoff, Mol. BioSyst., 2012, 8, 1861
    DOI: 10.1039/C2MB25086A

Search articles by author