Jump to main content
Jump to site search

Issue 7, 2012
Previous Article Next Article

Metabolic profiling of an alcoholic fatty liver in zebrafish (Danio rerio)

Author affiliations

Abstract

Zebrafish (Danio rerio) is becoming a popular developmental biology model to study diseases and for drug discovery. In this study, we performed proton nuclear magnetic resonance spectroscopy (1H-NMR)- and gas chromatography–mass spectrometry (GC/MS)-based metabolic profiling of an alcoholic fatty liver using a zebrafish disease model. We examined metabolic differences between the control and alcoholic fatty liver groups in zebrafish to determine how metabolism in an alcoholic fatty liver is regulated. Multivariate statistical analysis showed a significant difference between the control and alcoholic fatty liver groups. The alcoholic fatty liver group showed increased excretion of isoleucine, acetate, succinate, choline, creatine, acetoacetate, 3-hydroxybutyrate (3HB), ethyl glucuronide (EtG), lactate/pyruvate ratio, fatty acids, and cholesterol, and decreased excretion of citrate, aspartate, tyrosine, glycine, glucose, alanine, betaine, and maltose. Metabolites identified in the fatty liver groups were associated with long-term alcohol consumption, which causes both oxidation–reduction (redox) changes and oxidative stress. This study suggests that global metabolite profiling in a zebrafish model can provide insights into the metabolic changes in an alcoholic fatty liver.

Graphical abstract: Metabolic profiling of an alcoholic fatty liver in zebrafish (Danio rerio)

Back to tab navigation

Supplementary files

Publication details

The article was received on 06 Mar 2012, accepted on 30 Mar 2012 and first published on 25 Apr 2012


Article type: Paper
DOI: 10.1039/C2MB25073J
Citation: Mol. BioSyst., 2012,8, 2001-2009
  •   Request permissions

    Metabolic profiling of an alcoholic fatty liver in zebrafish (Danio rerio)

    Z. Jang, H. Chung, Y. G. Ahn, Y. Kwon, J. Kim, J. Ryu, D. H. Ryu, C. Kim and G. Hwang, Mol. BioSyst., 2012, 8, 2001
    DOI: 10.1039/C2MB25073J

Search articles by author

Spotlight

Advertisements