Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 4, 2012
Previous Article Next Article

A mass spectrometry-based targeted metabolomics strategy of human blastocoele fluid: a promising tool in fertility research

Author affiliations

Abstract

Embryo assessment is currently performed through the analysis of morphology and cleavage rate. Recent studies have sought to identify a correlation between quali-quantitative profiles of small molecules of metabolic interest and the outcome of embryo transfer. Approaches relying on both optical and non-optical spectroscopy have been proposed to non-invasively monitor the embryo culture media. However, the non-invasive approach only offers an indirect strategy to monitor embryos and a turn-around solution to bypass the limits of detection of these analytical techniques. In this paper we pave the way for direct metabolic assessment of embryos through the mass-spectrometry-based analysis of blastocoele fluid, which is withdrawn from the blastocoele cavity prior to cryostorage of blastocysts. We conclude that it is possible to detect most of the metabolites of potential interest right at the very heart of the blastocyst, without disrupting the workflow of a classic laboratory pipeline.

Graphical abstract: A mass spectrometry-based targeted metabolomics strategy of human blastocoele fluid: a promising tool in fertility research

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 02 Sep 2011, accepted on 28 Sep 2011 and first published on 21 Oct 2011


Article type: Method
DOI: 10.1039/C1MB05358B
Citation: Mol. BioSyst., 2012,8, 953-958
  •   Request permissions

    A mass spectrometry-based targeted metabolomics strategy of human blastocoele fluid: a promising tool in fertility research

    A. D'Alessandro, G. Federica, S. Palini, C. Bulletti and L. Zolla, Mol. BioSyst., 2012, 8, 953
    DOI: 10.1039/C1MB05358B

Search articles by author