Jump to main content
Jump to site search

Issue 2, 2012
Previous Article Next Article

Hydrophilic interaction and reversed-phase ultraperformance liquid chromatography TOF-MS for serum metabonomic analysis of myocardial infarction in rats and its applications

Author affiliations

Abstract

An ultra performance liquid chromatography coupled to mass spectrometry-based metabonomic approach, which utilizes both reversed-performance (RP) chromatography and hydrophilic interaction chromatography (HILIC) separations, has been developed to characterize the global serum metabolic profile associated with myocardial infarction (MI). The HILIC was found necessary for a comprehensive serum metabonomic profiling, providing complementary information to RP chromatography. By combining with partial least squares discriminant analysis, 21 potential biomarkers in rat serum were identified. To further elucidate the pathophysiology of MI, related metabolic pathways have been studied. It was found that MI was closely related to disturbed sphingolipid metabolism, phospholipid catabolism, fatty acid transportation and metabolism, tryptophan metabolism, branched-chain amino acids metabolism, phenylalanine metabolism, and arginine and proline metabolism. With the presented metabonomic method, we systematically analyzed the therapeutic effects of Traditional Chinese Medicine Sini decoction (SND). The results demonstrated that SND administration could provide satisfactory effects on MI through partially regulating the perturbed metabolic pathways.

Graphical abstract: Hydrophilic interaction and reversed-phase ultraperformance liquid chromatography TOF-MS for serum metabonomic analysis of myocardial infarction in rats and its applications

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 03 Aug 2011, accepted on 21 Sep 2011 and first published on 31 Oct 2011


Article type: Paper
DOI: 10.1039/C1MB05324H
Citation: Mol. BioSyst., 2012,8, 548-556
  •   Request permissions

    Hydrophilic interaction and reversed-phase ultraperformance liquid chromatography TOF-MS for serum metabonomic analysis of myocardial infarction in rats and its applications

    G. Tan, Z. Lou, W. Liao, X. Dong, Z. Zhu, W. Li and Y. Chai, Mol. BioSyst., 2012, 8, 548
    DOI: 10.1039/C1MB05324H

Search articles by author