Jump to main content
Jump to site search

Issue 21, 2012
Previous Article Next Article

Expanding 3D geometry for enhanced on-chip microbubble production and single step formation of liposome modified microbubbles

Author affiliations

Abstract

Micron sized, lipid stabilized bubbles of gas are of interest as contrast agents for ultra-sound (US) imaging and increasingly as delivery vehicles for targeted, triggered, therapeutic delivery. Microfluidics provides a reproducible means for microbubble production and surface functionalisation. In this study, microbubbles are generated on chip using flow-focussing microfluidic devices that combine streams of gas and liquid through a nozzle a few microns wide and then subjecting the two phases to a downstream pressure drop. While microfluidics has successfully demonstrated the generation of monodisperse bubble populations, these approaches inherently produce low bubble counts. We introduce a new micro-spray flow regime that generates consistently high bubble concentrations that are more clinically relevant compared to traditional monodisperse bubble populations. Final bubble concentrations produced by the micro-spray regime were up to 1010 bubbles mL−1. The technique is shown to be highly reproducible and by using multiplexed chip arrays, the time taken to produce one millilitre of sample containing 1010 bubbles mL−1 was ∼10 min. Further, we also demonstrate that it is possible to attach liposomes, loaded with quantum dots (QDs) or fluorescein, in a single step during MBs formation.

Graphical abstract: Expanding 3D geometry for enhanced on-chip microbubble production and single step formation of liposome modified microbubbles

Back to tab navigation

Supplementary files

Publication details

The article was received on 01 Jun 2012, accepted on 28 Aug 2012 and first published on 29 Aug 2012


Article type: Paper
DOI: 10.1039/C2LC40634A
Citation: Lab Chip, 2012,12, 4544-4552
  •   Request permissions

    Expanding 3D geometry for enhanced on-chip microbubble production and single step formation of liposome modified microbubbles

    S. A. Peyman, R. H. Abou-Saleh, J. R. McLaughlan, N. Ingram, B. R. G. Johnson, K. Critchley, S. Freear, J. A. Evans, A. F. Markham, P. L. Coletta and S. D. Evans, Lab Chip, 2012, 12, 4544
    DOI: 10.1039/C2LC40634A

Search articles by author

Spotlight

Advertisements