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Issue 17, 2012
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A paper and plastic device for performing recombinase polymerase amplification of HIV DNA

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Abstract

Despite the importance of early diagnosis and treatment of HIV, only a small fraction of HIV-exposed infants in low- and middle-income countries are tested for the disease. The gold standard for early infant diagnosis, DNA PCR, requires resources that are unavailable in poor settings, and no point-of-care HIV DNA test is currently available. We have developed a device constructed of layers of paper, glass fiber, and plastic that is capable of performing isothermal, enzymatic amplification of HIV DNA. The device is inexpensive, small, light-weight, and easy to assemble. The device stores lyophilized enzymes, facilitates mixing of reaction components, and supports recombinase polymerase amplification in five steps of operation. Using commercially available lateral flow strips as a detection method, we demonstrate the ability of our device to amplify 10 copies of HIV DNA to detectable levels in 15 min. Our results suggest that our device, which is designed to be used after DNA extraction from dried-blood spots, may serve in conjunction with lateral flow strips as part of a point-of-care HIV DNA test to be used in low resource settings.

Graphical abstract: A paper and plastic device for performing recombinase polymerase amplification of HIV DNA

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Publication details

The article was received on 27 Apr 2012, accepted on 31 May 2012 and first published on 01 Jun 2012


Article type: Paper
DOI: 10.1039/C2LC40423K
Citation: Lab Chip, 2012,12, 3082-3088
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    A paper and plastic device for performing recombinase polymerase amplification of HIV DNA

    B. A. Rohrman and R. R. Richards-Kortum, Lab Chip, 2012, 12, 3082
    DOI: 10.1039/C2LC40423K

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