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Issue 14, 2012
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In vitro and in vivo testing of glucose-responsive insulin-delivery microdevices in diabetic rats

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Abstract

We have developed glucose-responsive implantable microdevices for closed-loop delivery of insulin and conducted in vivo testing of these devices in diabetic rats. The microdevices consist of an albumin-based bioinorganic membrane that utilizes glucose oxidase (GOx), catalase (CAT) and manganese dioxide (MnO2) nanoparticles to convert a change in the environmental glucose level to a pH stimulus, which regulates the volume of pH-sensitive hydrogel nanoparticles and thereby the permeability of the membrane. The membrane is integrated with microfabricated PDMS (polydimethylsiloxane) structures to form compact, stand-alone microdevices, which do not require tethering wires or tubes. During in vitro testing, the microdevices showed glucose-responsive insulin release over multiple cycles at clinically relevant glucose concentrations. In vivo, the microdevices were able to counter hyperglycemia in diabetic rats over a one-week period. The in vitro and in vivo testing results demonstrated the efficacy of closed-loop biosensing and rapid response of the ‘smart’ insulin delivery devices.

Graphical abstract: In vitro and in vivo testing of glucose-responsive insulin-delivery microdevices in diabetic rats

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Publication details

The article was received on 08 Feb 2012, accepted on 30 Mar 2012 and first published on 08 May 2012


Article type: Paper
DOI: 10.1039/C2LC40139H
Citation: Lab Chip, 2012,12, 2533-2539
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    In vitro and in vivo testing of glucose-responsive insulin-delivery microdevices in diabetic rats

    M. K. L. Chu, J. Chen, C. R. Gordijo, S. Chiang, A. Ivovic, K. Koulajian, A. Giacca, X. Y. Wu and Y. Sun, Lab Chip, 2012, 12, 2533
    DOI: 10.1039/C2LC40139H

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