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Issue 17, 2012
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Microscopy imaging and quantitative phase contrast mapping in turbid microfluidic channels by digital holography

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Abstract

We show that sharp imaging and quantitative phase-contrast microcopy is possible in microfluidics in flowing turbid media by digital holography. In fact, in flowing liquids with suspended colloidal particles, clear vision is hindered and cannot be recovered by any other microscopic imaging technique. On the contrary, using digital holography, clear imaging is possible thanks to the Doppler frequency shift experienced by the photons scattered by the flowing colloidal particles, which do not contribute to the interference process, i.e. the recorded hologram. The method is illustrated and imaging results are demonstrated for pure phase objects, i.e. biological cells in microfluidic channels.

Graphical abstract: Microscopy imaging and quantitative phase contrast mapping in turbid microfluidic channels by digital holography

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Publication details

The article was received on 02 Feb 2012, accepted on 14 May 2012 and first published on 15 May 2012


Article type: Paper
DOI: 10.1039/C2LC40114B
Citation: Lab Chip, 2012,12, 3073-3076
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    Microscopy imaging and quantitative phase contrast mapping in turbid microfluidic channels by digital holography

    M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli and P. Ferraro, Lab Chip, 2012, 12, 3073
    DOI: 10.1039/C2LC40114B

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