Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 12, 2012
Previous Article Next Article

Frequency discretization in dielectrophoretic assisted cell sorting arrays to isolate neural cells

Author affiliations

Abstract

We present an automated dielectrophoretic assisted cell sorting (DACS) device for dielectric characterization and isolation of neural cells. Dielectrophoretic (DEP) principles are often used to develop cell sorting techniques. Here we report the first statistically significant neuronal sorting using DACS to enrich neurons from a heterogeneous population of mouse derived neural stem/progenitor cells (NSPCs) and neurons. We also study the dielectric dispersions within a heterogeneous cell population using a Monte-Carlo (MC) simulation. This simulation model explains the trapping behavior of populations as a function of frequency and predicts sorting efficiencies. The platform consists of a DEP electrode array with three multiplexed trapping regions that can be independently activated at different frequencies. A novel microfluidic manifold enables cell sorting by trapping and collecting cells at discrete frequency bands rather than single frequencies. The device is used to first determine the percentage of cells trapped at these frequency bands. With this characterization and the MC simulation we choose the optimal parameters for neuronal sorting. Cell sorting experiments presented achieve a 1.4-fold neuronal enrichment as predicted by our model.

Graphical abstract: Frequency discretization in dielectrophoretic assisted cell sorting arrays to isolate neural cells

  • This article is part of the themed collection: Focus on USA
Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 01 Dec 2011, accepted on 09 Mar 2012 and first published on 30 Mar 2012


Article type: Paper
DOI: 10.1039/C2LC21184J
Citation: Lab Chip, 2012,12, 2182-2189
  •   Request permissions

    Frequency discretization in dielectrophoretic assisted cell sorting arrays to isolate neural cells

    J. L. Prieto, J. Lu, J. L. Nourse, L. A. Flanagan and A. P. Lee, Lab Chip, 2012, 12, 2182
    DOI: 10.1039/C2LC21184J

Search articles by author