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Issue 8, 2012
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Immunosensor towards low-cost, rapid diagnosis of tuberculosis

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Abstract

A rapid, accurate tuberculosis diagnostic tool that is compatible with the needs of tuberculosis-endemic settings is a long-sought goal. An immunofluorescence microtip sensor is described that detects Mycobacterium tuberculosis complex cells in sputum in 25 minutes. Concentration mechanisms based on flow circulation and electric field are combined at different scales to concentrate target bacteria in 1 mL samples onto the surfaces of microscale tips. Specificity is conferred by genus-specific antibodies on the microtip surface. Immunofluorescence is then used to detect the captured cells on the microtip. The detection limit in sputum is 200 CFU mL−1 with a success rate of 96%, which is comparable to PCR.

Graphical abstract: Immunosensor towards low-cost, rapid diagnosis of tuberculosis

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Publication details

The article was received on 18 Nov 2011, accepted on 25 Jan 2012 and first published on 27 Jan 2012


Article type: Technical Innovation
DOI: 10.1039/C2LC21131A
Citation: Lab Chip, 2012,12, 1437-1440
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    Immunosensor towards low-cost, rapid diagnosis of tuberculosis

    J. Kim, W. Yeo, Z. Shu, S. D. Soelberg, S. Inoue, D. Kalyanasundaram, J. Ludwig, C. E. Furlong, J. J. Riley, K. M. Weigel, G. A. Cangelosi, K. Oh, K. Lee, D. Gao and J. Chung, Lab Chip, 2012, 12, 1437
    DOI: 10.1039/C2LC21131A

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