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Issue 6, 2012
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Microfluidic nanoplasmonic-enabled device for multiplex DNA detection

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Abstract

We describe a rapid, quantitative, multiplex, self-labelled, and real-time DNA biosensor employing Ag nanoparticle-bound DNA hairpin probes immobilized in a microfluidic channel. Capture of complementary target DNAs by the microarrayed DNA hairpin probes results in a positive fluorescence signal via a conformational change of the probe molecules, signalling the presence of target DNAs. The device's capability for quantitative analyses was evaluated and a detection time as low as 6 min (with a target flow rate of 0.5 μl min−1) was sufficient to generate significant detection signals. This detection time translates to merely 3 μl of target solution consumption. An unoptimized sensitivity of 500 pM was demonstrated for this device.

Graphical abstract: Microfluidic nanoplasmonic-enabled device for multiplex DNA detection

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Publication details

The article was received on 15 Nov 2011, accepted on 11 Jan 2012 and first published on 13 Jan 2012


Article type: Paper
DOI: 10.1039/C2LC21114A
Citation: Lab Chip, 2012,12, 1089-1093
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    Microfluidic nanoplasmonic-enabled device for multiplex DNA detection

    H. Peng, C. M. Strohsahl and B. L. Miller, Lab Chip, 2012, 12, 1089
    DOI: 10.1039/C2LC21114A

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