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Issue 17, 2012
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Microfluidic sorting of arbitrary cells with dynamic optical tweezers

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Abstract

Optical gradient forces generated by fast steerable optical tweezers are highly effective for sorting small populations of cells in a lab-on-a-chip environment. The presented system can sort a broad range of different biological specimens by an automated optimisation of the tweezer path and velocity profile. The optimal grab positions for subsequent trap and cell displacements are estimated from the intensity of the bright field image, which is derived theoretically and proven experimentally. We exhibit rapid displacements of 2 μm small mitochondria, yeast cells, rod-shaped bacteria and 30 μm large protoplasts. Reliable sorting of yeast cells in a microfluidic chamber by both morphological criteria and by fluorescence emission is demonstrated.

Graphical abstract: Microfluidic sorting of arbitrary cells with dynamic optical tweezers

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Publication details

The article was received on 11 Nov 2011, accepted on 29 May 2012 and first published on 30 May 2012


Article type: Paper
DOI: 10.1039/C2LC21099A
Citation: Lab Chip, 2012,12, 3177-3183
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    Microfluidic sorting of arbitrary cells with dynamic optical tweezers

    B. Landenberger, H. Höfemann, S. Wadle and A. Rohrbach, Lab Chip, 2012, 12, 3177
    DOI: 10.1039/C2LC21099A

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