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Issue 6, 2012
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Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements

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Abstract

Nanofluidic sensing elements have been the focus of recent experiments for numerous applications ranging from nucleic acid fragment sizing to single-molecule DNA sequencing. These applications critically rely on high measurement fidelity, and methods to increase resolution are required. Herein, we describe fabrication and testing of a nanochannel device that enhances measurement resolution by performing multiple measurements (>100) on single DNA molecules. The enhanced measurement resolution enabled length discrimination between a mixture of λ-DNA (48.5 kbp) and T7 DNA (39.9 kbp) molecules, which were detected as transient current changes during translocation of the molecules through the nanochannel. As long DNA molecules are difficult to resolve quickly and with high fidelity with conventional electrophoresis, this approach may yield potentially portable, direct electrical sizing of DNA fragments with high sensitivity and resolution.

Graphical abstract: Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements

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Publication details

The article was received on 17 Aug 2011, accepted on 20 Dec 2011 and first published on 02 Feb 2012


Article type: Paper
DOI: 10.1039/C2LC20771K
Citation: Lab Chip, 2012,12, 1094-1101
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    Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements

    Y. Sen, T. Jain, C. A. Aguilar and R. Karnik, Lab Chip, 2012, 12, 1094
    DOI: 10.1039/C2LC20771K

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