Issue 2, 2012

Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance genemecA in bacteria

Abstract

We present a high-throughput roll-to-roll (R2R) manufacturing process for foil-based polymethyl methacrylate (PMMA) chips of excellent optical quality. These disposable, R2R hot embossed microfluidic chips are used for the identification of the antibiotic resistance genemecA in Staphylococcus epidermidis. R2R hot embossing is an emerging manufacturing technology for polymer microfluidic devices. It is based on continuous feeding of a thermoplastic foil through a pressurized area between a heated embossing cylinder and a blank counter cylinder. Although mass fabrication of foil-based microfluidic chips and their use for biological applications were foreseen already some years ago, no such studies have been published previously.

Graphical abstract: Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance genemecA in bacteria

Article information

Article type
Paper
Submitted
19 Aug 2011
Accepted
08 Nov 2011
First published
29 Nov 2011

Lab Chip, 2012,12, 333-339

Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance genemecA in bacteria

R. Liedert, L. K. Amundsen, A. Hokkanen, M. Mäki, A. Aittakorpi, M. Pakanen, J. R. Scherer, R. A. Mathies, M. Kurkinen, S. Uusitalo, L. Hakalahti, T. K. Nevanen, H. Siitari and H. Söderlund, Lab Chip, 2012, 12, 333 DOI: 10.1039/C1LC20782B

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