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Issue 15, 2012
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A microfluidic “baby machine” for cell synchronization

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Abstract

Common techniques used to synchronize eukaryotic cells in the cell cycle often impose metabolic stress on the cells or physically select for size rather than age. To address these deficiencies, a minimally perturbing method known as the “baby machine” was developed previously. In the technique, suspension cells are attached to a membrane, and as the cells divide, the newborn cells are eluted to produce a synchronous population of cells in the G1 phase of the cell cycle. However, the existing “baby machine” is only suitable for cells which can be chemically attached to a surface. Here, we present a microfluidic “baby machine” in which cells are held onto a surface by pressure differences rather than chemical attachment. As a result, our method can in principle be used to synchronize a variety of cell types, including cells which may have weak or unknown surface attachment chemistries. We validate our microfluidic “baby machine” by using it to produce a synchronous population of newborn L1210 mouse lymphocytic leukemia cells in G1 phase.

Graphical abstract: A microfluidic “baby machine” for cell synchronization

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Publication details

The article was received on 19 Mar 2012, accepted on 20 Apr 2012 and first published on 24 Apr 2012


Article type: Paper
DOI: 10.1039/C2LC40277G
Citation: Lab Chip, 2012,12, 2656-2663
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    A microfluidic “baby machine” for cell synchronization

    J. Shaw, K. Payer, S. Son, W. H. Grover and S. R. Manalis, Lab Chip, 2012, 12, 2656
    DOI: 10.1039/C2LC40277G

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