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Issue 18, 2012
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Programmable large area digital microfluidic array with integrated droplet sensing for bioassays

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Abstract

We describe a new device concept for digital microfluidics, based on an active matrix electrowetting on dielectric (AM-EWOD) device. A conventional EWOD device is limited by the number of electrical connections that can be made practically, which restricts the number and type of droplet operations. In an AM-EWOD, the patterned electrodes of a conventional EWOD device are replaced by a thin film transistor (TFT) array, as found in a liquid crystal display (LCD), facilitating independent control of each electrode. The arrays can have many thousand individually addressable electrodes, are fully reconfigurable and can be programmed to support multiple simultaneous operations. Each element is 210 μm × 210 μm in size and contains a circuit that measures the electrical impedance of the liquid above it. This is used to determine the presence and size of a droplet, a method that can improve assay reliability and accuracy. This sensor provides feedback, error detection and closed loop control of an assay sequence. We describe the design, fabrication and testing of a 64 × 64 format AM-EWOD device with impedance sensor functionality. A colorimetric assay is implemented on the device and used to measure glucose in human blood serum. Results are compared with the same assay performed on a microtitre plate.

Graphical abstract: Programmable large area digital microfluidic array with integrated droplet sensing for bioassays

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Publication details

The article was received on 19 Mar 2012, accepted on 24 May 2012 and first published on 25 May 2012


Article type: Paper
DOI: 10.1039/C2LC40273D
Citation: Lab Chip, 2012,12, 3305-3313
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    Programmable large area digital microfluidic array with integrated droplet sensing for bioassays

    B. Hadwen, G. R. Broder, D. Morganti, A. Jacobs, C. Brown, J. R. Hector, Y. Kubota and H. Morgan, Lab Chip, 2012, 12, 3305
    DOI: 10.1039/C2LC40273D

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